标题：Study of Herbal Topical Repellent Made of Betel Leaves (Piper betle) and Patchouli Oil (Pogostemon cablin) Mixture Against Yellow Fever Mosquito (Aedes aegypti)
摘要：Introduction: Exploration of plants as natural repellants to avoid Aedes aegypti mosquitoes that transmit dengue virus to human is worthy . Betel vine leaves ( Piper betle L.) could be used as the material of repellant. The study is aimed to test the repellency , potency, and the safety of Piper betel and patchoulli oil modified gel against Aedes aegypti mosquitoes. Methods: This study is an experimental study and used nulliparous (3-5 days old) female Aedes aegypti . Before the study, safety test w as done which consist s of an i rritation test. The effectivity test used five treatments : DEET gel for positive control, piper and patchouli mixture gel, piper only gel, patchouli only gel and gel base only. The five treatments were tested as a repellant with five repetitive applications. The effectiveness of Aedes aegypti ’s rejection from biting the arms was analyzed using protective percentage. Results: The irritation test showed no safety concerns. Protective percentage analysis shows that modified betel vine gel has estimated mean protective power of over 90% for 6 hours . The data showed that the modified gels has about the same protective percentage as the DEET (ratio: PP (Neem+piper)/PP (DEET) =0.98; 95% confidence interval 0.93, 1.04; p-value=0.50) Conclusion Betel vine oil with modified formulation has a potential to be utilized as Aedes aegypti mosquito repellant and is safe to be used in further repellency test s .
