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  • 标题:Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
  • 本地全文:下载
  • 作者:Jie-Bi Hu ; Ting-Ru Chen ; Yu-Chie Chen
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2015
  • 卷号:5
  • DOI:10.1038/srep08135
  • 出版社:Springer Nature
  • 摘要:In order to ascertain optimum conditions for biocatalytic processes carried out in vitro , we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
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