Gaming industry is the pillar of Macao’s economy. The content of the gaming industry has become more extended and dynamic due to Mainland China policies and strategies of Macao government in tourism industry. As a leisure tourism destination with the gaming industry in Asia, Macao has become a popular tourist destination. This study tries to explore the impacts of cultural differences on gaming behaviors and reports on the observations of a convenience sample of 196 tourists in Chinese and non-Chinese. The results show that the nationality variable had a significant effect on the gambling, entertainment, shopping, and Food and beverage behaviors. The researchers provide some suggestions to government officers and gaming industry operators.