期刊名称：IRANIAN JOURNAL OF ENVIRONMENTAL HEALTH SCIENCE AND ENGINEERING (IJEHSE)
印刷版ISSN：1735-1979
出版年度：2010
卷号：7
期号：4
页码：313-318
语种：English
出版社：IRANIAN ASSOCIATION OF ENVIRONMENT HEALTH, TEHRAN, UNIVERSITY OF MEDICAL SCIENCES
摘要：The aim of this study was to isolate and characterize a high efficiency denitrifier bacterium for reducing nitrate in wastewater. Six denitrifier bacteria with nitrate removal activities were isolated from a petrochemical industry effluent with high salinity and high nitrogen concentrations without treatment. The isolated bacteria were testedfor nitrate reomoval activity. One of the bacterium displayed the highest reduction of nitrate. The strain was preliminarily identified using biochemical tests and further identified based on similarity of PCR-16S rRNA using universal primers. Biochemical and molecular experiments showed that the best bacterium with high nitrate removal potential was Pseudomonas stutzeri, a member of the a subclass of the class Proteobacteria. The extent of nitrate removal efficiency was 99% at 200 mg/L NO3 and the nitrite content of the effluent was in the prescribed limit. The experiments showed the ability of Pseudomonas stutzeri to rapidly remove nitrate under anoxic conditions. The strain showed to be potentially good candidate for biodenitrification of high nitrate solutions.
