期刊名称：Iranica Journal of Energy and Environment (IJEE)
印刷版ISSN：2079-2115
电子版ISSN：2079-2123
出版年度：2010
期号：3131
页码：132-136
出版社：Noshirvani University of TEchnology
摘要：In the present research, whey was used as useful substrate which retained from permeates of dairy industry. The obtained whey was hydrolyzed to cleave its main carbon source, lactose to glucose and galactose.The hydrolyzed products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Azohydromonas lata DSMZ 1123. The biosynthesis of PHA copolyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate were investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared to utilization of pure sugars. Therefore, fermentation under controlled conditions (agitation rate of 250 rpm, 18 h incubated inoculum, 72 h fermentation time and temperature set at 30°C) was performed. As a result, maximum amount biopolymer obtained was1.66 g/L. The biopolymer consisted 1.21 and 0.45 g/L of P(3HB) and P(3HV), respectively.
