标题：Production of Mycophenolic Acid by Penicillium brevicompactum in a Submerged Batch Culture: Optimization of Culture Conditions Using Taguchi Approach
期刊名称：Iranica Journal of Energy and Environment (IJEE)
印刷版ISSN：2079-2115
电子版ISSN：2079-2123
出版年度：2013
期号：3152
页码：330=335
出版社：Noshirvani University of TEchnology
摘要：Mycophenolic acid (MPA) is an antibiotic and immunosuppressive agent, as a secondary metabolite of Penicillium brevicompactum. Parameter optimization of culture composition using Test plan L16, available in the form of an orthogonal array and software for automatic design and analysis of the experiments, both based on Taguchi approach was performed in MAP production process by Penicillium brevicompactum ATCC 16024 in a batch submerged culture. Optimal levels of key parameters including glucose, enzymaticaly hydrolyzed casein, methionine and glycine concentrations were determined. Optimum glucose, enzymaticaly hydrolyzed casein, methionine and glycine concentrations were obtained 80, 25, 1.2 and 20 g. L 1, respectively. Theoretically expected and also experimentally actual obtained mycophenolic acid concentrations under the optimal conditions were 2.025 and 1.995 g. L 1, respectively with a good consistency as 98.5%. Analysis showed the glucose concentration was found to be the most significant factor as well as glycine concentration was the less important factor on MPA production in the investigated process.
