出版社：State Institution “O.M. MARZEYEV INSTITUTE FOR PUBLIC HEALTH OF THE NAMS OF UKRAINE"
摘要：Actuality of the work lies in the significance of the standardization of probiotic preparations. The standard samples of probiotic microorganisms are used for their standardization. Determination of standard samples’ safety is a compulsory condition. Materials and methods. We used generally accepted methods of the assessment of the microorganisms’ safety. Assessment of the safety of probiotic strains was conducted in acute and chronic experiments of the toxicity by the presence of lethal effect and change of the indices characterizing the functional state of the organism and separate organs of the experimental animals – mouse and rats. Results. During the testing of the strains B. bifidum and E. faecium at their single introduction into the organism of the animals (intraperitoneal, intragastric and intravenous) we failed to determine a main parameter of toxicity - an indicator of median lethal dose LD50. At the multiple introduction of the suspensions of probiotic strains B. bifidum and E. faecium into the organism the structural and functional changes in tissues and organs of experimental animals were not fixed, it indicates the strains’ safety. Thus, a safety of the microbial populations in the created standard samples B. bifidum and E. faecium was demonstrated in the models of acute and chronic experiments.
