摘要：Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile ( Cn -AFP), to prevent or delay ice formation. To coat Al with Cn -AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn -AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn -AFP-coated Al ( Cn -AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn -AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface–coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.
