文章基本信息

标题：Compare Two Contrasting Breeds of Pigs Postmortem for Differential Protein Expression in Relation to Meat Quality
本地全文：下载
作者：Daofeng Qu ; Jianzhong Han ; Kaili Jia 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2015
卷号：9
期号：8
页码：626-632
出版社：MAXWELL Science Publication
摘要：The aim of this study was to assess the postmortem changes of muscle proteins and their relationships between these changes and meat quality traits. Six Jinhua and 6 crossbred (Duroc×Landrace×Yorkshire) male pigs at 180 d were used to determine the post-mortem changes of meat during the storage. Differential proteins between Jinhua pigs and crossbred pigs were profiled and identified by the application of two-dimensional electrophoresis and Mass Spectrometry. Two-dimensional electrophoresis results showed that the expression level of 27 proteins from different breeds of pigs varied during postmortem time (6, 24, 48 and 72 h) and 16 of them was up-regulated in Jinhua pigs (p0.05). Furthermore, the structure and function of 12 proteins were identified by the application of Mass Spectrometry and referring to the protein database. Analysis showed that heat shock protein 27, desmin, β-enolase and pyruvate kinase were highly correlated with the integrity of meat tenderness, water-holding capacity as well as shelf life.
关键词：Differential proteomics ; meat quality ; pig ; postmortem changes ; storage ;