To compare central corneal thickness (CCT) as measured using noncontact specular microscopy (NCSM), dual rotating Scheimpflug camera (Galilei®), and ultrasound pachymetry (USP).

The measurements of CCT using NCSM, dual rotating Scheimpflug camera and USP in 70 eyes of 70 healthy subjects were compared.

The average measurements of CCT using NCSM, dual rotating Scheimpflug camera, and USP were 567.70 ± 31.21 µm, 557.84 ± 26.29 µm, and 553.31 ± 29.69 µm, respectively. The CCT measurement using NCSM was statistically significantly thicker than when measured using USP ( p < 0.05). There was no significant difference between the NCSM and dual rotating Scheimpflug camera ( p = 0.138). Additionally, there was no significant difference between the dual rotating Scheimpflug camera and USP ( p = 0.656). A significant linear correlation was observed among the NCSM, dual rotating Scheimpflug camera, and USP (r > 0.900, p < 0.001).

The results of the 3 methods were significantly correlated but the measurement using NCSM was significantly thicker than when using USP. CCT measurements of healthy eyes using dual rotating Scheimpflug camera were more correlated with USP than NCSM. The CCT measurements using dual rotating Scheimpflug camera is a better alternative for USP than NCSM.