摘要:We have isolated Pseudomonas sp. bacteria from Rangamati, Paschim Midnapur, West Bengal, India (La-22.27865 0 N, Lo-87.37988 0 E) which is predominantly lateritic in nature. It was screened for plant growth promoting (PGP) factors like phosphate solubilization, production of IAA, ammonia production and cell wall degrading enzyme activity (cellulose and protease) and growth inhibition against fungal and bacterial pathogens. The antibiotic test showed that isolate was susceptibility to amikacin, cotrimoxazole, chloramphenicol, tetracycline, vancomycin, ofloxacin, oxacillin, and gentamicin. It was found to be promising on all accounts. This work was carried out to explain the release of soluble phosphates and its effect on plant growth as a function of phosphate solubilization by fluorescent Pseudomonas sp. In the current study, It solubilized phosphate at a concentration of (175 μg/ml ) in Pikovskaya's (PVK) broth after 4 days of culturing. The strain produced a fluorescent blue-green pigment. Effect of temperature (4-60 0 C) and P H (5-9) on this strain were also looked into. The biochemical characterization of the strain was done. Inoculation of chickpea and paddy rice seeds with the isolate significantly enhanced seed germination, plant root weight, plant height, and also fresh and dry weight in comparison with the control. The study suggested an uniqueness of this isolated strain and its potentiality as in developing a low cost eco-friendly multifunctional bio-fertilizer and bio-control agent in agriculture with high plant growth promoting activity.
关键词:Pseudomonas sp.; PGP; phosphate solubilization; Oryza sativa L.