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  • 标题:Molecular method for identification of fallow venison
  • 本地全文:下载
  • 作者:Marušić Radovčić, Dr. sc. Nives ; Brekalo, dipl.ing., Ana ; Janči, dipl. ing., Tibor
  • 期刊名称:MESO The first Croatian meat journal
  • 印刷版ISSN:1332-0025
  • 出版年度:2015
  • 卷号:XVII
  • 期号:4
  • 页码:368-370
  • 语种:English
  • 出版社:ZADRUŽNA ŠTAMPA, d.d.
  • 摘要:Reliable and simple identification of species origin of meat and meat products can be carried out by applying species-specific primers that target particular short DNA fragments. The aim of our research was to design species-specific primers for the amplification of mitochondrial DNA control region of fallow deer (Dama dama) and to optimize the polymerase chain reaction protocol (PCR protocol) for such primers. Primers are used for the identification of fallow deer and the differentiation of fallow deer from other species, using only DNA extraction, PCR and electrophoresis. The application of primers was tested on eight fallow deer samples, three red deer and three roe deer samples.
  • 关键词:primer; mitochondrial DNA; fallow deer; Dama dama
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