文章基本信息
- 标题：Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts
- 本地全文：下载
- 作者：Hwang, Seok Jin ; Kim, Keun Hae
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2015
- 卷号：56
- 期号：12
- 页码：1939-1944
- DOI：10.3341/jkos.2015.56.12.1939
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts.
MethodsPrimary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 µg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining.
ResultsAME decreased significantly in fibroblast proliferation after exposure to 10 µg/mL ( p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 µg/mL ( p = 0.024).
ConclusionsAME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.
- 关键词：Amniotic membrane extract; Apoptosis; Nasal mucosa fibroblast
