期刊名称：International Journal of Environmental Science and Development
印刷版ISSN：2010-0264
出版年度：2013
卷号：4
期号：1
页码：37-43
DOI：10.7763/IJESD.2013.V4.299
摘要：Reliable methods are needed to measure and correlate bioavailable metals with aquatic toxicity.This research develops a method to measure bioavailable copper in estuarine waters using the cation exchange resin Chelex 100®.The Chelex 100® method performed consistently at copper concentrations from 195-495 μg/L when organic matter, pH and salinity were held constant.Varying salinity from 15-30 ppt did not affect measured bioavailable copper.As expected, an increase in pH from 4 to 8.5 and increase in NOM from 0 to 12.5 mg/L reduced measured bioavailable copper.Acute toxicity bioassays were performed with mysid shrimp (Americamysis bahia) and copper in the presence of NOM.At 20 ppt salinity, 48-hour LC50 dissolved copper concentrations were 200, 340, and 495 μg/L at 0, 12, and 24 mg/L NOM, respectively.The corresponding 48-hour LC50 values for bioavailable copper were nearly constant: 94, 98, and 105 μg/L Cu at 0, 12, and 24 mg/L NOM respectively.The consistency of the mysid shrimp LC50 values for bioavailable copper measured using the Chelex-100® method indicates that this method is appropriate for evaluating metal bioavailability in saline waters.
