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  • 标题:Isolation and Molecular Identification of an Aerobic Denitrifier
  • 本地全文:下载
  • 作者:Hongyu Wang ; Kai Yang ; Bin Ji
  • 期刊名称:International Journal of Environmental Science and Development
  • 印刷版ISSN:2010-0264
  • 出版年度:2013
  • 卷号:4
  • 期号:5
  • 页码:525-528
  • DOI:10.7763/IJESD.2013.V4.407
  • 摘要:Six bacteria strains of which total nitrogen removal efficiency is over 70% were isolated from the activated sludge after enrichment culture.The strain Z31 with higher nitrogen removal was selected and its characteristic of aerobic denitrification was confirmed by the nitrogen element track.The results showed that the nitrate in the culture could be efficiently removed by strain Z31 and the nitrate nitrogen removal rate was up to above 95%.There was obvious nitrite accumulation during the denitrification process.Moreover, medium pH was increased and medium ORP was decreased as a result of denitrification.According to the morphological observation, physiological biochemical test and sequence analysis of the 16S rDNA, strain Z31 was identified as Pseudomonas stutzeri.And the phylogentic position of the strain was performed based on the phylogenetic tree.
  • 关键词:Aerobic denitrifier; biological nitrogen removal; nitrate wastewater; Pseudomonas stutzeri
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