Biofuels for transport belong to a sector, which functions in a volatile global environment (macro). Until the end of 2010, European Union had promoted the production and development of vegetable based biofuels for transport as a way to reduce the emission of greenhouse gases (nonobligatory Directive 2003/30/EC, with indicator of replacing 5.75% of all transport fossil fuels with biofuels in 2010, reached in about 80%). Currently, bio-components with high CO₂ emission reduction and biofuels produced from inedible plants and raw material waste are being promoted. The Directive 2009/28/EC, which has been in force since 2011, has imposed mandatory obligation on all subjects, who participate in the cycle of biofuels and bioliquid production, to fulfill criteria of sustainable production, including CO₂ emission reduction to at least the threshold level (e.g. at least 50% reduction by 2017). In the article, rating method for CO₂ reduction in the international law setting have been presented – BIOGRACE 4 calculator and value of CO₂ emission reduction in five plants, where biofuels are produced with use of three methods.