期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：2
页码：239-243
DOI：10.5604/1232-1966.1108583
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. RLB (Reverse Line Blot Hybridization) is a molecular biology technique that might be used for Borreliaburgdorferi sensu lato (sl) DNA detection with genospecies specification. Among B. burgdorferi sl genospecies at least 7 areregarded as pathogenic in Europe.Objective. The aim of the study was to evaluate the frequency of different Borrelia genospecies DNA detection in Ixodesricinus ticks in the endemic area of North-Eastern Poland by using RLB.Materials and method. Ixodes ricinus ticks were collected in May – June, from 6 different sites in North-Eastern Poland(Jakubin, Kolno, Grajewo, Suwałki, Siemiatycze, Białowieża) by flagging. Extracted DNA was amplified by polymerasechain reaction (PCR) targeting the intergenic spacer 5S 23S of B. burgdorferi sl. PCR products were hybridised to15 different oligonucleotide probes for 9 different Borrelia genospecies (B. burgdorferi sl, B. burgdorferi ss, B. garinii,B. afzelii, B. valaisiana, B. lusitaniae, B. spielmanii, B. bissettii and B. relapsing fever-like spirochetes (B. myamotoi)) by RLB.Results. Borrelia genospecies DNA was detected in 205 Ixodes ricinus ticks. Among 14 infected with Borrelia ticks, 4 wereidentified as B. garinii and 10 as B. afzelii. Higher numbers of infected ticks were noticed in the eastern part of the researcharea, where large forest complexes dominate. Nymphs appeared to be the most frequently infected tick stage, which hasan epidemiological meaning in the incidence of Lyme borreliosis.Conclusions. The study demonstrated that RLB might be easily used in Borrelia DNA detection with genospecies-identification,and indicated the domination of B. afzelii and B. garinii in ticks from North-Eastern Poland
