期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：2
页码：249-255
DOI：10.5604/1232-1966.1108585
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Spray-application of membraneless acidic electrolyzed water (MLAEW) is a novel technique for disinfectionin livestock houses. This study investigated the loss of free chlorine (FC – the major germicidal component in MLAEW) overdistance during spraying, as affected by air temperature and initial FC concentration. The anti-microbial effect of MLAEWon airborne bacteria from an aviary laying-hen house was examined.Materials and methods. MLAEW was prepared at two FC concentrations: app. 15 and 60 mg L-1, and sprayed at three airtemperatures (18, 25, 32 °C). The original MLAEW solution and MLAEW aerosols collected at 0, 25, and 50 cm from the spraynozzle were analyzed for FC concentrations. Bacteria were immersed into these MLAEW samples and numerated for viablecount after 0.5, 2 and 5-min treatments.Results. MLAEW aerosols collected at 0 cm lost 11.7–13.2% FC, compared with the original MLAEW solution. This initial losswas affected neither by the initial FC concentration (P = 0.13) nor by air temperature (P = 0.57). The rate of FC loss duringtravelling was 0.79–0.87 % per cm of aerosol travel distance (% cm-1) at 18 °C, 1.08–1.15 % cm-1 at 25 °C, and 1.35–1.49% cm-1 at32 °C. This travelling loss was affected by air temperature (P = 0.02), but not by initial FC concentration (P = 0.38). Bacteria werecompletely inactivated at 0.5 min when treated with MLAEW samples with FC > 16.8 mg L-1, in 2 min when FC > 13.8 mg L-1,and in 5 min when FC > 7.2 mg L-1.Conclusion. Airborne bacteria from aviary hen house can be effectively inactivated by MLAEW with adequate FC concentrationand contact time. During spraying, the anti-microbial efficacy of MLAEW aerosols decreased over distance due to FC losswhich exacerbated at higher air temperatures
