期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：2
页码：256-258
DOI：10.5604/1232-1966.1108586
出版社：Institute of Agricultural Medicine in Lublin
摘要：Objective. The aim of this study was to estimate the content of peptidoglycans in cutting fluids (CFs) and to assess thepossibility of using them as a marker of bacterial contamination in this type of occupational environment.Materials and methods. A total of 11 samples of CFs were collected: 8 were taken from the working machine systems and3 were unused CF samples. The peptidoglycans were determinated with the kinetic version of the Silkworm Larvae Plasma(SLP) test.Results. The average concentration of bacteria was 5.58×105 CFU/mL, and peptidoglycans – 28.2 ng/mL. The variabilityfor peptidoglycans concentration was less pronounced than that for bacteria (GSD 6 and 13.3, respectively). Taking intoconsideration the National Research and Safety Institute (INRS – Institut National de Recherche et de Sécurité) limit value theconcentrations of bacteria and peptidoglycans, as well as the usage of the fluids, the analysis showed that peptidoglycansreflect the differences between the studied factors much more accurately than bacteria. The correlation analysis, however,showed that the levels of peptidoglycans in the examined CFs strongly correlated with the concentrations of viable bacteria(R2 = 0.50, p<0.05).Conclusions. The study confirmed that the CFs may contain immunologically active substances of bacterial origin eventhough they did not show any bacterial growth. Moreover, it showed that the concentrations of peptidoglycans in CFsprecisely reflect the exposure to bacteria, and as a structural component of the cell wall can be treated as their marker
关键词：bacterial contamination; cutting fluids; peptidoglycans; SLP test
