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  • 标题:Detection of TBEV RNA in Ixodes ricinus ticks in north-eastern Poland
  • 本地全文:下载
  • 作者:Beata Biernat ; Stella Cieniuch ; Joanna Stańczak
  • 期刊名称:Annals of Agricultural and Environmental Medicine
  • 印刷版ISSN:1232-1966
  • 电子版ISSN:1898-2263
  • 出版年度:2014
  • 卷号:21
  • 期号:4
  • 页码:689-692
  • DOI:10.5604/12321966.1129915
  • 出版社:Institute of Agricultural Medicine in Lublin
  • 摘要:AbstractTBEV (Flaviviridae, Flavivirus) is an etiologic agent of tick-borne encephalitis (TBE), the most important arboviral humaninfection involving the central nervous system. The disease is endemic in a zone extending from central and eastern Europeto Siberia and Japan, and corresponds to the distribution of the ixodid ticks, which act both as the vectors and reservoirof TBEV. Humans acquire infection mainly by the bite of an infected tick. A continuous increase of TBE cases throughoutEurope has been observed over a period of 30 years. The objective of this study was a preliminary determination of theinfection level of ticks collected in north-eastern Poland, the endemic area of TBE. Questing I. ricinus ticks (adults, nymphsand larvae) were collected by flagging the lower vegetation in 55 locations in Poland in 2006–2009. A total of 2,075 ticks(676 females, 555 males, 799 nymphs and 45 larvae) were collected and examined for the presence of RNA TBEV by nestedRT-PCR. The average number of ticks in one pool was 6.98. The minimum infection rate of ticks with TBEV was estimatedin the total area as 0.96%. TBEV RNA was detected in all of the investigated developmental stages of ticks. The prevalenceof viral infection in ticks is a useful indicator of TBE virus circulation, and may be used for risk assessment of the degree ofnatural focus activity and of the risk to contact TBE in a particular natural habitat
  • 关键词:Ixodes ricinus; TBEV; tick borne encephalitis virus; nRT-PCR; Poland
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