期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：4
页码：730-732
DOI：10.5604/12321966.1129924
出版社：Institute of Agricultural Medicine in Lublin
摘要：Leptospira is an important re-emerging zoonotic human pathogen, disseminated by sick and carrier animals, water and soil.Weather calamities, such as flooding or cyclones favour the spreading of these bacteria. To check a potential role of naturalwater and soil in the persistence and spread of Leptospira on the territory of eastern Poland, 40 samples of natural waterand 40 samples of soil were collected from areas exposed to flooding, and 64 samples of natural water and 68 samples ofsoil were collected from areas not exposed to flooding. Samples of water were taken from various reservoirs (rivers, naturallakes, artificial lakes, canals, ponds, farm wells) and samples of soils were taken at the distance of 1–3 meters from the edgeof the reservoirs. The samples were examined for the presence of Leptospira DNA by nested-PCR. Two out of 40 samplesof water (5.0%) collected from the area exposed to flooding showed the presence of Leptospira DNA, while all 40 samplesof soil from this area were negative. All samples of water and soil (64 and 68, respectively) collected from the areas notexposed to flooding were negative. No significant difference were found between the results obtained in the areas exposedand not exposed to flooding. In conclusion, these results suggest that water and soil have only limited significance in thepersistence and dissemination of Leptospira in eastern Poland.
