期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：1
页码：69-75
DOI：10.5604/12321966.1141371
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction and objective. Exposure to conidia of Aspergillus fumigatus was described as a causative factor of a numberof the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitisand bronchopulmonary aspergillosis. The study investigates the effects of the repeated exposure to A. fumigatus inmice pulmonary compartment. Our work tackles two, so far insufficiently addressed, important aspects of interactionbetween affected organism and A. fumigatus: 1) recurrent character of exposure (characteristic for pathomechanism ofthe abovementioned disease states) and 2) impact of aging, potentially important for the differentiation response to anantigen.Materials and methods. In order to dissect alterations of the immune system involved with both aging and chronicexposure to A. fumigatus, we used 3- and 18-month-old C57BL/6J mice exposed to repeated A. fumigatus inhalations for 7and 28 days. Changes in lung tissue were monitored by histological and biochemical evaluation. Concentration of pro- andanti-inflammatory cytokines in lung homogenates was assessed by ELISA tests.Results and conclusions. Our study demonstrated that chronic inflammation in pulmonary compartment, characterizedby the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response torepeated A. fumigatus inhalations. The pattern of cytokines’ profile in the course of exposure was similar in both age groups,however in old mice the growth of the cytokines’ levels was more pronounced (especially in case of IL1).
关键词：Aspergillus fumigatus; inflamm-aging; cytokines’ profile; chronic exposure; mouse model
