标题：Detection of protozoans Babesia microti and Toxoplasma gondii and their co-existence in ticks (Acari: Ixodida) collected in Tarnogórski district (Upper Silesia, Poland)
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：1
页码：80-83
DOI：10.5604/12321966.1141373
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Ticks (Acari: Ixodida) are vectors of many pathogens i. a: Babesia microti [Bm] and Toxoplasma gondii [Tg]. InPoland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are asignificant threat to human and animal health.Objectives of the study. Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area.Material and methods. The ticks were collected from vegetation and pets in the spring period of their activity on theterritory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method.Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit wasused. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light.Results. It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% ofall examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted.Conclusions. The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role inthe transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tgmay indicate their important role in the transmission of this pathogen, but it requires a further studies.
