期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：2
页码：227-230
DOI：10.5604/12321966.1152070
出版社：Institute of Agricultural Medicine in Lublin
摘要：Acanthamoeba is an opportunistic protozoan pathogen which is widely distributed in nature and plays a pivotal role in theecosystem. Acanthamoeba species may cause blinding keratitis and fatal granulomatous encephalitis involving the centralnervous system. This study investigates the presence of Acanthamoeba in soil and water resources in Pakistan. Acanthamoebawere recovered on non-nutrient agar plate lawn with E. coli and identified by morphological characteristics of the cyst. PCRwas performed with genus-specific primers, followed by direct sequencing of the PCR product for molecular identification.Overall, the PCR and sequencing results confirmed pathogenic genotypes, including T4 and T15, from both soil and watersamples. This is the first report of Acanthamoeba isolation from both soil and water resources in Pakistan which may serveas a potential threat to human health across the country
关键词：Acanthamoeba; genotyping; soil resources; water resources; PCR; Pakistan
