期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：2
页码：325-328
DOI：10.5604/12321966.1152088
出版社：Institute of Agricultural Medicine in Lublin
摘要：In alcoholic liver cirrhosis, normal liver cells are replaced by scar tissue (fibrosis). Liver fibrosis is a dynamic process in whichactivated hepatic stellate cells are involved in the synthesis of matrix proteins and the regulation of matrix degeneration.The aim of the presented study was to assess the usefulness of MMP-2, MMP-8 and MMP-9 as diagnostic markers of alcoholicliver disease. Sixty patients with alcoholic liver cirrhosis were randomly enrolled during hospitalization in departments ofhospitals from the Lublin Region in eastern Poland. The stage of cirrhosis was estimated according to Child-Turcotte-Pughcriteria (Child-Pugh score) as P- Ch A, P-Ch B, P-Ch C. The control group consisted of 10 healthy persons without liver disease,who did not drink alcohol. Additionally, a group of alcoholics without liver cirrhosis was included in the study. Blood samplewere obtained, and after centrifuge, serum was collected for further analysis. The activity of MMP-2, MMP-8 and MMP-9in the blood plasma of the patients and the control group were measured by using the sandwich enzyme immunoassaytechnique with commercially available quantitative ELISA test kits. Activity of MMP-2, MMP-8 and MMP-9 in patients withliver cirrhosis were increased gradually according to Child-Pugh stages. The activity of MMP-2, MMP-8, MMP-9 were thehighest in patients with liver cirrhosis stage C. MMP-2, MMP-8, MMP-9 concentrations in the people with liver cirrhosis(stage C) were significantly increased compared to controls. A significant difference were observed between activity MMP-2in control group, alcoholics without liver cirrhosis, and those with liver cirrhosis (stages A, B, C according Child-Pugh score).MMP-2, MMP-8 and MMP-9 may be markers of alcoholic liver cirrhosis in the alcoholics. Elevated levels of MMP-2, MMP-8 andMMP-9 in the alcoholic patients indicated that cirrhosis has developed. The most sensitive is MMP-2, because the activity ofthis parameter is increased in all liver cirrhosis stages. MMP-8 and MMP-9 activity were significantly elevated only in serumpatients with advanced liver cirrhosis, compared to controls
