期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：3
页码：433-437
DOI：10.5604/12321966.1167708
出版社：Institute of Agricultural Medicine in Lublin
摘要：Objective. The objective of the study was measurement of serum arachidonic acid level as well as the product of itsperoxidation – 8-isoPGF2a, and the activity of phospholipase A2 and PAF-acetylhydrolase that participate in releasing8-isoPGF2a from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD).Material and methods. Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF2a were determinedin the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A2 and PAFacetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) afterantibiotics treatment.Results. An almost 3-fold higher level of the total plasma 8-isoPGF2a was observed in LA patients compared to the controls,while in the urine it increased over 5-fold. The plasma PLA2 activity was more than 3-fold higher in LA patients than in thehealthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total8-isoPGF2a level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasmaactivity of PAF-AH in the LA patients was increased, while the cPLA2 activity decreased after antibiotics treatment.Conclusions. It may be suggested that in the course of LA, the level of binding 8-isoPGF2a is significantly enhanced, andit may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritisunresponsive to antibiotics
