期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：4
页码：690-694
DOI：10.5604/12321966.1185777
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Alzheimer’s disease is a progressively developing neurodegenerative disorder of the central nervoussystem. The only present treatment of this disease is the use of acetyl- and butyrylcholinesterase inhibitors. Previously, theneuroprotection of phenolic acids and flavonoids in the brain has been indicated.Materials and method. This study measured anticholinesterase activities of 9 phenolic acids and 6 flavonoids, singly orin combination. The synergy/antagonism/zero interaction between compounds was evaluated taking into considerationthe statistical significance. Ellman’s modified spectrophotometric method was used with the simultaneous measurementof the false-positive effect of compounds.Results. The anti-acetylcholinesterase activity of phenolic acids was as follows: homogentisic acid > 4-hydroxyphenylpyruvicacid > nordihydroguaiaretic acid > rosmarinic acid > caffeic acid > gallic acid = chlorogenic acid > homovanillic acid > sinapicacid. p-Hydroxyphenylpyruvic, caffeic, chlorogenic, gentisic, homogentisic, nordihydroguaiaretic and rosmarinic acids inpairs exhibited, in most cases, a lower inhibitory activity (at p>0.05), than the sum of the activities of single compounds. Also,phenolic acids in pairs with flavonoids (cyanidin, delphinidin, kaempferol, myricetin, phloridzin, pelargonidin or quercetin)presented, in most cases, a lower inhibitory activity than could be calculated for both compounds singly (at p>0.05). Onlyin the case of a few samples was the inhibitory activity of two compounds higher than the sum of inhibitions exerted bythe same compounds tested singly (either at p>0.05 or p<0.05). The lack of synergy of pairs of inhibitors suggests one smallbinding site, making impossible to accommodate both inhibitors adjacent to one another
