摘要：The oxidation resistance gene 1 ( OXR1 ) is crucial for protecting against oxidative stress; however, its molecular function is unknown. We employed RNA sequencing to examine the role of human OXR1 for genome wide transcription regulation. In total, in non-treated and hydrogen peroxide exposed HeLa cells, OXR1 depletion resulted in down-regulation of 554 genes and up-regulation of 253 genes. These differentially expressed genes include transcription factors (i.e. HIF1A , SP6, E2F8 and TCF3 ), antioxidant genes ( PRDX4 , PTGS1 and CYGB ) and numerous genes of the p53 signaling pathway involved in cell-cycle arrest (i.e. cyclin D , CDK6 and RPRM ) and apoptosis (i.e. CytC and CASP9 ). We demonstrated that OXR1 depleted cells undergo cell cycle arrest in G2/M phase during oxidative stress and increase protein expression of the apoptosis initiator protease CASP9. In summary, OXR1 may act as a sensor of cellular oxidative stress to regulate the transcriptional networks required to detoxify reactive oxygen species and modulate cell cycle and apoptosis.
