首页    期刊浏览 2024年11月24日 星期日
登录注册

文章基本信息

  • 标题:D-Xylose as a sugar complement regulates blood glucose levels by suppressing phosphoenolpyruvate carboxylase (PEPCK) in streptozotocin-nicotinamide-induced diabetic rats and by enhancing glucose uptake in vitro
  • 本地全文:下载
  • 作者:Kim, Eunju ; Kim, Yoo-Sun ; Kim, Kyung-Mi
  • 期刊名称:Nutrition Research and Practice
  • 印刷版ISSN:1976-1457
  • 出版年度:2016
  • 卷号:10
  • 期号:1
  • 页码:11-18
  • DOI:10.4162/nrp.2016.10.1.11
  • 语种:English
  • 出版社:KoreaMed Synapse
  • 摘要:BACKGROUND/OBJECTIVES

    Type 2 diabetes (T2D) is more frequently diagnosed and is characterized by hyperglycemia and insulin resistance. D-Xylose, a sucrase inhibitor, may be useful as a functional sugar complement to inhibit increases in blood glucose levels. The objective of this study was to investigate the anti-diabetic effects of D-xylose both in vitro and stretpozotocin (STZ)-nicotinamide (NA)-induced models in vivo .

    MATERIALS/METHODS

    Wistar rats were divided into the following groups: (i) normal control; (ii) diabetic control; (iii) diabetic rats supplemented with a diet where 5% of the total sucrose content in the diet was replaced with D-xylose; and (iv) diabetic rats supplemented with a diet where 10% of the total sucrose content in the diet was replaced with D-xylose. These groups were maintained for two weeks. The effects of D-xylose on blood glucose levels were examined using oral glucose tolerance test, insulin secretion assays, histology of liver and pancreas tissues, and analysis of phosphoenolpyruvate carboxylase (PEPCK) expression in liver tissues of a STZ-NA-induced experimental rat model. Levels of glucose uptake and insulin secretion by differentiated C2C12 muscle cells and INS-1 pancreatic β-cells were analyzed.

    RESULTS

    In vivo , D-xylose supplementation significantly reduced fasting serum glucose levels ( P < 0.05), it slightly reduced the area under the glucose curve, and increased insulin levels compared to the diabetic controls. D-Xylose supplementation enhanced the regeneration of pancreas tissue and improved the arrangement of hepatocytes compared to the diabetic controls. Lower levels of PEPCK were detected in the liver tissues of D-xylose-supplemented rats ( P < 0.05). In vitro , both 2-NBDG uptake by C2C12 cells and insulin secretion by INS-1 cells were increased with D-xylose supplementation in a dose-dependent manner compared to treatment with glucose alone.

    CONCLUSIONS

    In this study, D-xylose exerted anti-diabetic effects in vivo by regulating blood glucose levels via regeneration of damaged pancreas and liver tissues and regulation of PEPCK, a key rate-limiting enzyme in the process of gluconeogenesis. In vitro , D-xylose induced the uptake of glucose by muscle cells and the secretion of insulin cells by β-cells. These mechanistic insights will facilitate the development of highly effective strategy for T2D.

  • 关键词:D-xylose; sugar-complement; Diabetes; PEPCK; Glucose uptake
国家哲学社会科学文献中心版权所有