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  • 标题:X-ray structure of the MMTV-A nucleosome core
  • 本地全文:下载
  • 作者:Timothy D. Frouws ; Sylwia C. Duda ; Timothy J. Richmond
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2016
  • 卷号:113
  • 期号:5
  • 页码:1214-1219
  • DOI:10.1073/pnas.1524607113
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar α-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-Å resolution X-ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3′-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg2+ ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove.
  • 关键词:chromatin ; nucleosome ; DNA ; X-ray structure ; MMTV
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