出版社：Japan Science and Technology Information Aggregator, Electronic
摘要：We investigated the effects of Japanese sake on the GABAA receptor which plays a crucial role in effecting relaxation in the central nervous system. The activities of the GABAA receptor channel were measured by using a Xenopus oocyte expression system. For removing ethanol and volatile compounds, two kinds of sake (junmai–shu) were fractionated by ion–exchange chromatography and lyophilized. The obtained four fractions consisted mainly of basic amino acids (C Fra.), neutral and acidic amino acids (R Fra.), organic acids (A Fra.) and sugars (N Fra.). The GABAA receptor was activated by all fractions of sake. In particular, GABA–free A Fra. highly activated the GABAA receptor. These results indicated that sake contains components able to activate the GABAA receptor except for ethanol and GABA.
