The newly proposed concept, the Regional Pattern for Ecological Security (RPES), provided an integrated strategy for implementing ecosystem restoration and biodiversity conservation at regional scale. Based on the concept and its theoretical basis, the designing principles and a preliminary method were put forward. With the method, the Regional Pattern for Ecological Security of the area along the Yangtze River in Anhui Province (the AYRAP) is constructed. In terms of the ecological features of the AYRAP, an ecological security pattern should be established, consisting of 3 ecological sources, 6 ecological corridors and numerous ecological patches and wedges.