From the economic perspective, training can be perceived as an enterprise’s act of investing in employees, and the post-training transfer effect is the return acquired by the enterprise. Obviously, training transfer provides a new perspective for research of the training effect and training return on investment of the enterprise. From three aspects including personal characteristics, training design, and transfer atmosphere, this paper designs a questionnaire to investigate the factors that affect training transfer of grassroots employees in petroleum enterprises. The empirical analysis has shown that the main factors affecting training transfer of grassroots employees in petroleum enterprises are personal characteristics, organizational support and management support.