摘要：Tylophora indica and Bacopa monnieri are two important medicinal plants containing several secondary metabolites of pharmaceutical value. To understand the biosynthetic pathways of these metabolites, molecular studies will be helpful. A prerequisite for these studies is good quality DNA that is free of contaminants. A number of DNA extraction protocols are available, but many of them require expensive and harmful chemicals. Thus, a protocol was developed to isolate genomic DNA from fresh leaf tissue of these plant species using minimum chemicals. The quantity of the isolated DNA was found to be 6200 μg mL –1 and 4925 μg mL –1 for T. indica and B. monnieri, respectively, with their A 260/280 ratios ~1.80. For both, the sample A 260/230 ratios were ~2.0 and A 260/270 ratios were ~1.2. Spectroscopic analysis, agarose gel electrophoresis and polymerase chain reaction revealed that the isolated DNA was suitable for carrying out genomic studies
关键词：Bacopa monnieri; DNA extraction; Tylophora indica
