摘要：A series of experiment had been done to evaluate the use of purified lignin formacell (PLF) and its derivative (MIL and MSL) as feed supplement and prebiotics candidate for ruminant. PLF was isolated from empty bunch palm fiber. First experiment was microbial test using PLF, MIL and MSL as substitute of glucose in medium. Six treatments were applied and growth of Lactobacillus casei , Lactobacillus bulgaricus and Streptococcus thermophillus were measured. Second experiment was also microbial test using PLF, MIL and MSL as substitute of yeast extract in medium. Six treatments were applied and growth of Escherichia coli , Salmonella typhimurium and Staphylococcus aureus were measured. Third experiment was in vitro test using fresh rumen liquid to study the following treatments: R0 = basal diet; R1 = R0 + 1.25% of Inulin; R2 = R0 + 1.25% of PLF; R3 = R0 + 1.25% of MIL and R4 = R0 + 1.25% of MSL. Microbial test showed that PLF, MIL, or MSL could substitute the glucose in the medium as carbon source s for the growth of lactic acid bacteria; whereas PLF, MIL, or MSL could not be used as carbon source s in the medium for growth of the pathogenic bacteria . In vitro rumen fermentation revealed that the addition of PLF, MIL, or MSL has positive effects on rumen metabolism parameters. Higher ammonia was achieved by MIL and MSL; higher rate of microbial protein synthesis was achieved by PLF and MIL and higher crude fiber digestibility was achieved by MIL and MSL.
