文章基本信息

标题：基于Nafion与硫堇离子交换行为的NADH生物传感器的构建与性能评价
其他标题：Construction and Evaluation of NADH Biosensor Based on Ion-Exchange Behavior between Nafion and Thionine
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作者：税月 ; 吉艳 ; 梁羽佳等
期刊名称：Journal of Sensor Technology and Application
印刷版ISSN：2331-0235
电子版ISSN：2331-0243
出版年度：2015
卷号：03
期号：03
页码：25-32
DOI：10.12677/JSTA.2015.33004
出版社：Hans Publishers
摘要：以Nafion为离子交换材料，通过离子交换的方法制备了硫堇(Thionine, TH)/有序介孔碳(Ordered mesoporous carbon, OMC)复合材料修饰玻碳电极(Glassy carbon electrode, GCE)，用电化学方法对该修饰电极进行了详细表征，在循环伏安曲线上可以观察到硫堇的一对可逆的氧化还原峰，证明硫堇已经被成功的固定到电极表面；对修饰电极的动力学研究表明电极过程是受表面控制。利用此修饰电极，0 V电位下即可以实现对烟酰胺腺嘌呤二核苷酸(Nicotinamide adenine dinucleotide, NADH)的安培检测，线性范围可达1 × 10⁻⁶ mol∙L⁻¹~6 × 10⁻⁴ mol∙L⁻¹，检出限达到1 × 10⁻⁷ mol∙L⁻¹ (信噪比为3)，并且能很好的消除尿酸、多巴胺以及对乙酰氨基酚(p-acetamidophenol, AP)的干扰，有希望成为有应用价值的NADH传感器。
其他摘要：Thionine/OMC/GCE was prepared by ion-exchange procedure. Electrochemical behaviors of the resulting electrode were investigated thoroughly with cyclic voltammetry, and a well-defined redox couple was clearly visualized. Under a lower operation potential of 0 V in 0.1 mol∙L−1 PBS (pH 7.0), NADH could be detected linearly up to a concentration of 1 × 10 ⁻⁶ mol∙L ⁻¹ ~6 × 10 ⁻⁴ mol∙L ⁻¹ mol∙L−1 with an extremely lower detection limit of 1 × 10 ⁻⁷ mol∙L ⁻¹ estimated (S/N = 3). The feasibility for simultaneous determination of uric acid, dopamine, AP and NADH was discussed. They did not in-terfere with each other in certain concentration. Based on the results, a new NADH sensor was successfully established using the PTH/OMC/GCE.
关键词：有序介孔碳 ; 电催化 ; 硫堇 ; NADH ; Ordered Mesoporous Carbon ; Electrocatalysis ; Thionine ; NADH
其他关键词：有序介孔碳 ; 电催化 ; 硫堇 ; NADH ; Ordered Mesoporous Carbon ; Electrocatalysis ; Thionine ; NADH