摘要:The present study provides updated information on amphipod assemblages of the southern Portuguese coast, the most south-western part of continental Europe. The influence of depth and sediment type on the spatial distribution of shallow soft-bottom amphipods was tested by means of univariate and multivariate methods. Samples were collected down to 40 m depth within areas ranging from very fine sand to gravel. The ratio between density and diversity was found to increase with depth and from coarser to finer sediments. Both physical variables tested were found to play a major role in structuring amphipod assemblages. However, some dominance of depth was evident as samples were mainly separated according to this variable. Moreover, while only 15 taxa were common to all depth levels, 35 were collected from all sediment types. Both depth and sediment type may override the potential effects of geographical location, as samples from different areas along the southern Portuguese coast were biologically related. A peculiar habitat was sampled in this study: a maerl bed, which was confirmed to be a hotspot of density. Nevertheless, this habitat did not harbor a distinct amphipod assemblage but some taxa such as Caprella spp., Lembos websteri , lysianassids and Gammarella fucicola were preferentially collected there. The biogeographical analysis pointed out the occurrence of 8% of Mediterranean endemic species, confirming the influence of the Mediterranean Sea beyond the Strait of Gibraltar, and the Atlantic-Mediterranean feature of the amphipod fauna of this geographically relevant area. The present results support the need to interpret the spatial distribution patterns of marine species in relation to the combined effect of depth and sediment characteristics.