期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：1
页码：18763
DOI：10.15680/IJIRSET.2015.0401057
出版社：S&S Publications
摘要：In vitro flowering and an efficient micropropagation protocol was developed for the annual medicinalherb Physalis minima (Solanaceae) by the in vitro culture of nodal segments of mature plant. Murashige and Skoog’s(MS)medium, 3% sucrose and0.8% agar supplemented with 4.0mg/l BAP and 0.25mg/l 2,4 - D and1.0mg/l KIN and0.25mg/l NAA were found to develop fully functional flowers. MS medium supplemented with 2.0mg/l BAP and0.25mg/l IAA induced an average of three shoots per explants. Full strength MS medium with 2.0mg/l IBA exhibitedthe best in in vitro rooting. Seventy percent of the rooted shoots survived when transferred to green house and next tothe field condition.
