期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：3
页码：868
DOI：10.15680/IJIRSET.2015.0403010
出版社：S&S Publications
摘要：Whey is a byproduct which contains nutritional value added proteins like, α-Lactalbumin, α-Lactoglobulin. Although several possibilities for cheese-whey exploitation have been assayed, approximately half of worldcheese-whey production is not treated, but is discarded as effluent. It is necessary to develop an effective andenvironmentally benign aqueous two phase system (ATPS) for the recovery of value added cheese whey proteins.Recently ATPS have drawn more interest among the other separation processes, particularly in the field ofbiotechnology due to the mild conditions of the process, short processing time, and ease of scale up. In order to designa two stage ATPS process the effect of system parameters such as pH, types and the concentrations of the phaseforming components , temperature, etc., on partitioning of proteins were addressed a view to maximizing recovery. Inthe present study, partitioning of whey proteins from dairy effluent by using polyethylene glycol and sodium sulphatewas done. The results showed that PEG-4000 at 50% concentration and salt at 40% concentration under conditions ofpH 5.4 and temperature 35ºC will give maximum yield and partition coefficient.
关键词：Cheese whey; aqueous biphasic system; Extraction; dairy effluent; Protein recovery.
