期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：3
页码：938
DOI：10.15680/IJIRSET.2015.0403021
出版社：S&S Publications
摘要：Biodegradation of acid blue 113 has been studied using two strains-Staphylococcus aureus andEscherichia coli. The effect of pH, time, initial dye concentration, the inoculum size, the co-substrate, static andshaking on decolourisation was studied. The optimum condition for decolourisation was found to be at pH 7 for aninitial dye concentration of 200 mg/L at 37 ̊C under static condition. The removal of dye by S.aureus and E.coli atoptimum condition was 90% and 88% within 30 h and 42 h respectively. Addition of fructose and starch as carbonsource increased the removal of colour by S.aureus and E.coli respectively. Similarly addition of ammonium formateand glycine as nitrogen source under static condition increased the colour removal by S.aureus and E. coli. Theenzyme activity of azoreductase for S. aureus and E. coli was found to be 7.33 U/ml and 7.24 U/ml respectively. TheCOD reduction, FTIR and TLC analysis of the sample before and after decolourisation confirmed the biodegradation ofdye and the formation of new metabolite. Phytotoxicity studies indicated that the microbial treated effluent is safe fordisposal into the environment.
