期刊名称:International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN:2347-6710
电子版ISSN:2319-8753
出版年度:2015
卷号:4
期号:4
页码:1870
DOI:10.15680/IJIRSET.2015.0404010
出版社:S&S Publications
摘要:The present work attempts to investigate the ability of fusariumoxysporum and neurosporacrassa toferment cellulose to ethanol directly. Batch experiments were performed for both the strain at 30.c in 250ml conicalflasks.1000ml of production medium was distributed equally in 7 sterile,250 ml conical flasks, each containing variousinitial concentrations of cellulose 20,30,40,50,60,70 and 80g/l respectively.20ml of inoculum medium was transferredto each 100ml production medium in sterile conditions. Initial values of ethanol concentration, cellulose concentrationand cell mass were determined. The inoculated flasks were maintained at 300 C in a rotary shaker operating at 200 revmin-1.Analyses were carried out for every 24 hours using the contents of the various conical flasks.Experiments wereconducted for mixed cultures of different compositions 80:20, 60:40, 40:60, and 20:80by volume respectively.Thecultures fusariumoxysporum and neurosporacrassa were mixed in proportion 80:20,60:40,40:60,and 20:80 respectivelymixed cultures were used for inoculation.Based on the kinetic models, equations have been developed for thedetermination cell mass, product and substrate concentration as a function of time. Using the experimental data,constants of these equations have been evaluated. These mathematical relationships can be used with reasonableaccuracy.
关键词:Cellulose; Ethanol; Mixed culture; Batch processes and Kinetic Studies.