期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：5
页码：2981
DOI：10.15680/IJIRSET.2015.0405066
出版社：S&S Publications
摘要：Various applications of Manganese Peroxidase such as transformation of dyes, biobleaching,biopulping, treating wastewater contaminated with phenols, chlorinated phenol & cresols enhances the interest forfurther study on the enzyme. The present aim of the study is to purify Manganese peroxidase from plant source Musaacuminata stem, an agro waste and to monitor its effect on effluent degradation. Manganese peroxidase was extractedfrom Musa acuminata stem juice using 0.1M Phosphate buffer, Ammonium Sulphate precipitation & Dialysistechniques were carried out to partially purify the enzyme. Column chromatography was packed with silica gel andchloroform with 0.1M Phosphate Buffer as elution buffer to achieve 10.8 fold purification with 32% enzyme yield,specific activity was found to be 24.5 U/mg. The Km and Vmax value with MnSO4 as variable substrate were calculatedfrom MM and double reciprocal plots. The pH and temperature of the enzyme were optimized. The FTIR analysis ofthe degraded effluents was analyzed.
