期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：6
页码：5039
DOI：10.15680/IJIRSET.2015.0406168
出版社：S&S Publications
摘要：An isocratic simple rapid method has been developed and validated for the determination ofcarbamazepine (CBZ) in both solution form and blood samples. The assay was performed using an analytical C18 (100mm×4.6 mm i. d) with a mobile phase consisting of methanol and water (50:50), the flow rate was 1 ml/min and UVdetection at 285 nm. The method was found to be specific for CBZ, no interfering peaks were observed with an overallanalytical run time of 5 min. Accuracy reported as % recovery were found to be 95.83–103.34% and 93.18–104.49%for inter-day and intra-day accuracies, respectively. Inter-day precision (reproducibility) was found to be 0.22–1.19SD, while intra-day precision (repeatability) was found to be 0.14–2.12 SD for the samples studied. The calibrationcurve was found to be linear with the equation y=13474x+16969, with a correlation coefficient of 0.997 (R2) over aconcentration range of 0.5–40 lg/ml. The farthest point of quantitation was the least fixation. The system isstraightforward and fast and does not require any preparatory treatment of the specimen. The strategy was approvedand effectively requested the blood tests to study the medication pharmacokinetic parameters.
