期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：8
页码：6773
DOI：10.15680/IJIRSET.2015.0408010
出版社：S&S Publications
摘要：Lubricant oil from Pump motors, automobile engines and engines of boats and ships constitute animportant pollutant of water and soil ecosystems, leading to devastating damage to the aquatic ecosystem at times ofaccidents. There are wide varieties of microorganisms in water and soil known to utilize petroleum hydrocarbons as anenergy source and degrade them. Hydrocarbon utilizers (HCUs) found to be useful in the remediation of oil pollution insoil and water, includes certain species of Pseudomonas, Rhodococcus, Bacillus. This work is primarily based ondegradation study of engine oil and evaluation of the effectiveness of indigenous microorganisms in the process.Microbes isolated from polluted sites have been screened for their ability to grow under in vitro culture, where usedengine oil was the sole carbon source. Evaluation of residual oil content and percentage degradation of oil in culturemedium was measured at regular intervals of 7 days for a period of 28 days. Residual oil was estimated by the toluenecold extraction method, percentage degradation of hydrocarbons was evaluated using Gas Chromatography (GC). Andheavy metals in the sample before and after remediation were also analyzed by Energy diffraction spectroscopy (EDS)and characterised using Scanning Electron microscope (SEM). Results of the study have indicated that microbes arepotential agents studied under in vitro conditions for restoring oil contamination and thereby biodegrading the harmfulhydrocarbon pollution
