期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：8
页码：6922
DOI：10.15680/IJIRSET.2015.0408181
出版社：S&S Publications
摘要：Background: Many Excipients are known to interact with P-gp and CYP enzyme system therebylimiting the oral bioavailability. The present study was aimed to investigate the influence of poloxamer 188(withdifferent concentrations) pretreatment on the intestinal transport of Diltiazem Hydrochloride.Methods: The transport of Diltiazemacross different parts of rat intestine was studied by everted and non-everted sacmethods. The control and poloxamer 188 (0.25%, 0.5% and 1% for 7 days) pre-treated rats were sacrificed and isolatedthe intestine. The sacs of intestine were prepared, treated with Diltiazemsolution and then placed in dulbeccos buffer.Samples were collected periodically and the drug content was estimated using HPLC.Results and conclusion: The results show that there was a significant (p<0.05) difference in the transport of Diltiazemfrom the intestinal sacs of pretreated with poloxamer 188 and control. Pretreatment with Poloxamer decreased drugexsorption in everted studies and increased transport in non everted studies compared to control indicating theinhibition of P-gp transporter and CYP3A enzyme respectively
关键词：Diltiazem; Poloxamer 188; Everted; Non Everted; Exsorption and High Performance Liquid;Chromatography method (HPLC).
