期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：8
页码：7001
DOI：10.15680/IJIRSET.2015.0408195
出版社：S&S Publications
摘要：Despite extensive research done on nuclear embryogenesis in citrus, a major snag remains unsolved thatthe nuclear embryo proliferation does not lead to production of even a fraction of the number of globular embryosformed initially. During further proliferation, mostly normal differentiation of embryos gets drowned in the mass ofproliferating cotyledonary structures. The present experimentation is mainly aimed to resolve this problem and producesufficient number of nucellar plantlets per culture. The significance of nucellar embryogenesis, particularly in case ofrootstocks can hardly be over emphasized. However, it is also significant in case of C. jambhiri, where commercial useof its nucellars for raising orchards is in practice. Nucellus is a somatic tissue having a genetic makeup similarly to thevegetative plant body. The culture of nucellar tissue and augmentation and induction of nucellar polyembryony haveinitially been the main focus of research. However, the associated extended juvenile characteristics of nucellarplantlets, if not slightly poor quality of fruit, restrict the application of in vitro nucellar embryogenesis to rootstocksonly, since both such characteristics will not be of any consequence while virus-free rootstocks will be produced at arapid rate throughout the year, which in turn is imperative for all the year round production of micrografts. Whencritically examined, the nucellar embryonal proliferation, i.e., embryo-to-embryo multiplication is an uncommonphenomenon. Instead, there is cotyledonary proliferation forming resette-like structures having a single radicle withpluricotyly, which is a big snag in production of nucellar plantlets.
