期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：9
页码：9116
DOI：10.15680/IJIRSET.2015.0409119
出版社：S&S Publications
摘要：Fluorescent proteins are genetically encoded, easily imaged reporters crucial in biology andbiotechnology. The fluorescent proteins from the jelly fish Aequorea Victoria, has become a versatile reporter formonitoring gene expression and protein localization in the variety of cells and organisms. The fluorescent proteins(cherry gene) can be introduced into Escherichia coli and maintained their genome through injection with a viral vectoror cell transformation. Transformants were obtained by the transformation procedure. After transformation, thebacterial transformants were confirmed using agarose gel electrophoresis. Normally the fluorescent proteins arehydrophobic in nature. So the cherry proteins were purified by hydrophobic interaction chromatography. Afterpurification, the amounts of fluorescent proteins were estimated by Bradfoed method. Finally the maximum absorbancevalue of fluorescent (cherry) proteins were identified. This would be identified by maximum gene expression of the(cherry) fluorescent protein.
关键词：Gene expression; protein localization; transformation; hydrophobicinteraction chromatography
