The aim of this study was to compare and examine the platelet distribution width (PDW) among highly physically active, moderate physically active and low physically active groups at 16 weeks of observation. Thirteen (13) SAI football students as highly physically active group (SAI), twelve (12) professional physical education students as moderate physically active group (B.P.Ed) and ten (10) general college going students as very low physically active group (GEN) who has normal blood cell count at the beginning of the session with an average age of 21.16±1.85 have participated in this study voluntarily. SAI and B.P.Ed groups were involved in their respective training schedule under the supervision of experts. The researcher observed the all the groups for 16 weeks and collected the data three times for every sample. Platelet distribution width (PDW) has been determined by using Syxmex autohemato analyzer. The result of the study found that in case of SAI trainees, statistically significant increase in platelet distribution width was evident after 2^nd month (q=4.28, p<0.01), but maintained the same level even after the 4^th month of training (q= 0.12, p>0.05). Similar improvement was evident among B.P.Ed trainees in Platelet distribution width after 2^nd month (q=3.29, p<0.05), but maintained in the normal level on the 4^th month of training (q= 0.14, p>0.05). Considerably, no significant change in platelet distribution width was evident in general students after 2^nd month (q=0.10, p>0.05) and even on 4^th month of training (q= 0.15, p>0.05). During 2^nd and 4^th months of training, SAI and B.P.Ed trainees maintained the platelet distribution width at a better state than the trainees of general students. Moreover, the SAI and B.P.Ed trainees maintained higher Mean platelet volume than the general students.