期刊名称：Studia Universitatis Moldaviae: Stiinte Exacte si Economice
印刷版ISSN：1857-2073
电子版ISSN：2345-1033
出版年度：2011
期号：6(46)
页码：51-57
出版社：Moldova State University
摘要：Phaseolin, the 7S seed storage protein from common beans (Phaseolus vulgaris L.), partially modified in vivoduring seed germination and seedling growth was isolated from cotyledons of 6 day germinated seedlings (phaseolininvivo).SDS-PAGE showed that the pattern of fragments of phaseolin-invivo is similar to that of the phaseolin degradedby legumain - phaseolin-LLP. Native electrophoresis showed that phaseolin-invivo consists of four molecularforms. 2-D electrophoresis showed that the molecular form of phaseolin-invivo that is similar to phaseolin-LLP retainsin its quaternary structure some of the fragments detected by SDS-PAGE. Phaseolin-invivo retains the property toreversibly associate/dissociate with the changes in pH. At pH 4.6 it is present in its dodecameric form while at pH 8.0 inits protomeric form. The implications of the finding that phaseolin-invivo retains the same property of native phaseolinto associate into dodecamers at acidic pHs are discussed
