期刊名称：Asian Journal of Biological and Life Sciences
印刷版ISSN：2278-747X
电子版ISSN：2278-5957
出版年度：2015
卷号：4
页码：114-121
出版社：Pbspublishing
摘要：Five agro-residues viz. sugarcane bagasse, sugarcane tops, sugarcane trash, corn husk and corn stover were alkali pretreated using 3.0% NaOH for 8 hours under ambient condition and then autoclaved at 1210C, 15 lbs pressure for 1h. The pretreated residues were subjected for saccharification using crude enzymes of four proven cellulolytic fungi at 10, 20 and 30 FPU per gram substrate. The release of reducing sugars as influenced by various concentrations of crude cellulase enzymes indicated that the treatment of 20U g-1 was optimum for all the crude enzyme extracts of different fungi studied. The crude enzyme extract of Trichoderma reesei when used at 20 U g-1 showed the significantly superior amounts of release of reducing sugars and per cent saccharification respectively in sugarcane bagasse (477.45 mg g-1 and 67.88%), sugarcane tops (428.68 mg g-1 and 62.94%), sugarcane trash (437.45 mg g-1 and 64.22%), corn husk (411.26 mg g-1 and 59.70%) and in corn stover (421.83 mg g-1 and 61.32%).
